BLOCK-iT™ Pol II miR RNAi Vector Services

BLOCK-iT? Pol II miR RNAi Vector ServicesDNA based or vector mediated RNA Interference (RNAi) is often used for long-term expression, hard to transfect cell lines or for inducible RNAi. Vector technologies allow you to:
Achieve transient or stable target knockdownPerform RNAi in any cell type – even hard to transfect, primary and non-diving cellsRegulate gene inhibition with inducible RNAi expressionStudy long-term gene knockdown
BLOCK-iT? Pol II miR RNAi vectors combine the benefits of traditional RNAi vectors – stable expression and the ability to use viral delivery – with capabilities for tissue-specific expression and multiple target knockdown from the same transcript. These vectors are designed to express artificial miRNAs that are engineered to have 100% homology to your target gene and result in target cleavage.
Table 1: BLOCK-iT? Pol II miR RNAi Entry VectorsmiR RNAi Entry Vector Advantages pcDNA?6.2- GW/miR Pol II CMV Promoter for constitutive, transient expressionPoly-cistronic miR RNAi expressionTransfer miR RNAi cassettes into other Gateway? pDEST? vectors including Lentiviral vectors pcDNA?6.2- GW/EmGFP- miR Co-cistronic EmGFP reporter for easy tracking of miR RNAi expressionPol II CMV Promoter for constitutive, transient expressionPoly-cistronic miR RNAi expressionTransfer miR RNAi cassettes into other Gateway? pDEST? vectors including Lentiviral vectors
Once your miR RNAi entry vector is generated, our RNAi services team will transfect it into mammalian cells to perform knockdown studies, chain multiple miR RNAi sequences together to knock down more than one target with the same vector, or transfer the miR RNAi sequence to another destination vector. Regardless of your vector choice, once expressed in a cell, the miR RNAi sequence induces an RNAi response resulting in knockdown of the targeted message.
Subcloning BLOCK-iT? Pol II miR RNAi SequencesThe BLOCK-iT? Pol II miR RNAi Subcloning Service includes a Gateway? BP and subsequent LR recombination reaction to move the miR RNAi sequence from the miR RNAi entry vector into the Gateway? destination vector of your choice. Currently there are many compatible destination vectors to choose from including lentiviral vectors and vectors with tissue specific promoters. Each destination vector has different features and benefits for increased flexibility in your experiments. We’ll help you choose the best destination vector that meets your experimental goals.
BLOCK-iT? Lentivirus ProductionFor many disease models the most desirable cell types to use, such as non-dividing or primary cells, cannot be efficiently transfected. Invitrogen’s lentiviral delivery system solves this problem by offering a powerful alternative to routine transfections. Lentiviral delivery has proven to be successful with a variety of cell types including:
Post mitotic or non-dividing cellsPrimary cellsStem cellsGrowth arrested cellsAnimal models
Once your BLOCK-iT? Pol II miR RNAi sequence is in a lentiviral destination vector, Invitrogen’s virus production team can produce either crude or concentrated lentiviral stocks in a variety of quantities and formats to meet your experimental goals.
BLOCK-iT? Pol II miR RNAi Phenotypic AssaysThe ultimate goal of gene knockdown is to observe changes in phenotype. Invitrogen’s Custom Services will work with you to design and execute a wide variety of phenotypic assays using BLOCK-iT? Pol II miR RNAi vectors.
BLOCK-iT? Pol II miR RNAi Custom ResearchInvitrogen’s team of expert scientists has years of experience working with RNAi and will work with you to design your RNAi experiments to reliably achieve your research goals. For more information on any of the BLOCK-iT? Pol II miR RNAi Custom Services listed here, please contact Invitrogen Custom Services.